- G. Verheyen one ,
- H. Tournaye ,
- C. Staessen ,
- A. De Vos ,
- M. Vandervorst and
- A. Van Steirteghem
- Free University of Brussels for Reproductive Medicine, Academic Hospital, Dutch-speaking, Laarbeeklaan 101, B-1090 Brussels, Belgium Center
- Received March 4, 1999.
- Accepted June 1, 1999.
Comparison was controlled between (ICSI) intracytoplasmic sperm injection and (IVF) is of = 5% rapid progressive (type) is being done in patients with sperm in the semen fresh in vitro fertilization traditional . You maybe interested in foods that increase sperm and volumethat. Twenty couples were assigned to the study. All semen samples met the minimum criteria to be assigned to the conventional in vitro fertilization. Check out also increase sperm fluid volume program. Fertilization rate (2PN) after in vitro fertilization has been completed to 10 points from 20 cycles, with failure of conception, (22.9%), than after in vitro fertilization traditional . You maybe interested in foods that increase sperm and volumepronuclei of two per cumulus-oocyte complex was obtained was significantly higher after (63.4%) ICSI also. Quality of the embryos, was similar for both treatments. 16 cases were subjected to two patients who were receiving in vitro fertilization and ICSI embryos and IVF embryos only patients at high ICSI, of two people. Ongoing clinical pregnancy rate was 45%, implantation rate was 37%. Revealed a high percentage of sperm fraction sperm and to compare the characteristics of the / cycle patients, regardless of the presence or absence of fertilization 2PN, exercise gradually, that has been prepared for a group of patients with fertilization after in vitro fertilization traditional . You maybe interested in foods that increase sperm and volume. It can be concluded from this study a very low percentage of rapid progressive movement of fresh semen or absent, indicates that the risk of fertilization failure in vitro fertilization of a complete conventional high.
Introduction in 1992 (since, from Palermo, 1992 ), intracytoplasmic sperm injection (ICSI) is efficient for the treatment of cases of infertility male factor serious ineligible for in vitro fertilization (IVF) of conventional has been applied worldwide as a technique. Quality and basic sperm parameters appeared to be unrelated to global results in terms of fertilization rate and pregnancy rate ( Mansour et al, 1995 ; Nagy et al, 1995. ; from Svalander, 1,996 ). In a prospective, controlled study, some of the sibling oocytes for the adaptation of different male and ICSI, we compared the effectiveness of the conventional in vitro fertilization. Check out also increase sperm fluid volume. (In some studies . Aboulghar et al, 1 995 , one thousand nine hundred and ninety-six ; . Calderon et al, 1995 ), the result was very supportive of the steps ICSI. Conventional in vitro fertilization. Check out also increase sperm fluid volume, in the latter study, was associated with almost 50% of the total failure of fertilization. See sperm volume increase. Other groups, in the case of severe teratozoospermia compared the in vitro fertilization and ICSI. According to the author of some, the problem of a high percentage of abnormal forms, traditional in vitro fertilization (for example, can be overcome by (HIC) "` high insemination concentration from Ord, 1993. ; from the Hall, 1995 ; from Oehninger, 1996. other), however, we recommend treatment ICSI versus IVF diagnosis of teratozoospermia or severe ICSI (. You maybe interested in sperm volume. from Mr. Payne, 1994 ; from Fishel, 1,995 . it) from the reason of andrological, IVF can be assumed that the selection criteria of IVF difference in efficiency between the ICSI, to depend on the number and quality of sperm for in vitro fertilization artificial insemination, and the performance of a program of IVF and ICSI and. See how to increase volume of sperm. in vitro fertilization traditional . You maybe interested in foods that increase sperm and volumeComparison of ICSI is valid, and can be done properly only if the criteria of sperm to allow the acceptance of the couple for in vitro fertilization treatment have been met.
It is pointed out that men can be processed correctly only in severe ICSI is clear. It is the difference in efficiency between the IVF and ICSI is theoretically, not very clear whether or not to exist for the case of men meet the criteria for the actual boundary of the conventional in vitro fertilization. Check out also increase sperm fluid volume. has been extensively studied is teratozoospermia, asthenozoospermia impact of boundary on the fertilization and embryo quality after conventional IVF and ICSI is not so clear. In the center of the most cut-off value for sperm preparation after the exercise, has been defined in order to determine the admittance of a couple of traditional in vitro fertilization program gradually. General (WHO, 1992) is used (in m / s May 25, five type B) sperm, but slow with movement gradually the sum of the minimum number of rapid (type A,> of 25m / s) and . To our center, this number is = 500. 000 + B-type sperm. The problem is, the presence of major slow progressive type B sperm, may indicate whether the contraindication for conventional in vitro fertilization. Check out also increase sperm fluid volume or ICSI adaptation will occur in the semen. You maybe interested in ginseng sperm volume.
The purpose of the research a prospective, controlled current, as a movement in the semen sample fresh = 5% type, in the case of asthenozoospermia defined in this study was to compare the efficiency of ICSI in vitro fertilization and traditional. View reduced sperm volume.
Most of the couple, based on the quality of the semen of diagnostic testing, assigned to the research during a visit to the outpatient setting. Semen characteristics will meet the following criteria. (1992,) that, (ii) = 500 000 for the entire sperm motility (type A + B) gradually motility of sperm fresh type of progressive rapid (i) = 5% which is a limitation of the quality of the sperm sample required for admission to the program in the conventional in vitro fertilization. Check out also increase sperm fluid volume center after preparation of the semen. Other semen parameters were not taken into account. On the day of oocyte retrieval, were included in the study of semen is only a couple again the above-mentioned conditions are met effectively. View increase volume of sperm load cumshot. Five couples were excluded from the study plan is for this reason on an outpatient basis. You maybe interested in largest sperm volume. 14 cases, during the other six patients have been assigned to research on the day of oocyte retrieval based on the quality of their sperm at that point in the context of both (a severe asthenozoospermia = who apparently was accepted) rapid progressive motility of 5%. Cumulus 8 - less than oocyte complex is if it was obtained in the (inclusion criteria die), the oocytes of all were treated with ICSI, patients also these, were excluded from the study acquisition of the eggs.
In total, were conducted (20 couples) for 20 cycles. The mean age of the patients (39 from 25 range) of 3.4 years for male partners of female partners and 35.4 4.9 years was 31.6 (45 from 27 range).Ovarian stimulation
Human menopausal gonadotropin in combination with (HMG), partners of women, ovarian stimulation was subjected to suppression protocol using gonadotropin-releasing hormone analogues buserelin (Hoechst, Frankfurt, Germany nasal spray Suprefact) and. Oocyte cumulus complexes were recovered by vaginal ultrasound-guided search for 36 hours after the administration of 10 000 IU of (HCG) human chorionic gonadotropin.Evaluation and preparation of semen
Fresh semen samples were provided by masturbation on the day of oocyte collection. After liquefaction, sperm concentration and motility were evaluated in accordance with the recommendations of the (1992) World Health Organization. Of sperm morphology, Kruger strict criteria (was assessed using Kruger et al, 1986 ). Semen samples from all patients included in the study showed a rapid progressive motility of = 5%. Percoll (95% -47.5%, Pharmacia, Uppsala, Sweden), but are based on, sperm (90% -45%, Institute Nidacon, Gothenburg, Sweden) replaced, after the first non-pure sample of all semen was prepared by continuous density gradient centrifugation. Fraction 1ml of fresh semen was placed in 1 ml gradient consisting of 1 ml min and the top of the bottom fraction. View sperm volume requirement for icsi. Gradient of more than one, was held as necessary. After centrifugation at 300 g for 20 minutes, two times before suction from 400l to evaluate the fraction of under 200, and washed. Per sperm (type A + B) after microliter concentration and motility has been evaluated, and then concentrated or diluted depending on the number of progressively motile one of this fraction.Acquisition and preparation of oocytes
Oocyte retrieval was performed by transvaginal ultrasound-guided puncture of follicles 36 hours after administration of 10 000 IU hCG. You maybe interested in how to increase sperm volume. Cumulus-oocyte complex first, had been assigned to conventional IVF or ICSI according to one of the randomization table. Subsequent cumulus - oocyte complex were assigned to ICSI and IVF further alternately. Those used for in vitro fertilization, were cultured in 25l droplets of B2 medium under paraffin oil separately.
Conventional in vitro fertilization. Check out also increase sperm fluid volume, oocyte, after you retrieve the eggs, (enter the A + B) has been fertilized by sperm movement in 5000 at least 2 hours gradually. Check out also increase sperm volume formula. You can add 5l volume was between 1 and. Volume was concentrated fractions sperm last, was calculated to provide cell motility 5000 exceeds the 5l, before the volume of semen is added, the medium is excessive, removed from the droplets of the first has been. Cumulus assigned to ICSI, oocyte complex, by exposure to hyaluronidase 10 IU / ml (type VIII, Sigma Chemical Co., St. Louis, MO, USA) was added to a solution of enzymatically followed by pipetting, has been stripped from cumulus cells. Was injected only mature, the oocyte-II mid-term. Care was taken to perform the injection of the same time of the day ICSI oocyte insemination of oocytes in IVF (around 14:00). You maybe interested in diet for high volume sperm. ICSI is a procedure (as described above was implemented Van Steirteghem et al, 1995 ).Fertilization, embryo quality and embryo transfer
Evaluation of fertilization, we performed about 18 hours after fertilization and injection of oocytes. Check out also minimum sperm volume requirement foe icsi. Was considered oocytes (2PN), usually with the fertilization of two separate pronuclei with fragmented polar body or two separate. In oocytes ICSI, the pronucleus is when you lost sometimes up to that time, the cleavage of the normal body and two poles has been observed, was considered oocytes are usually, and fertilization. Normal fertilization rate, egg hill as always for ICSI - was calculated as the number of greater than or equal to the number of 2PN for in vitro fertilization, as well as complex independent of nuclear maturation of oocytes. Inseminated from 42 to 44 hours after injection, quality and development speed of embryo morphology was evaluated. Embryos were classified into different categories in proportion to the fragment: Enter the embryos without Hai fragment, of type B fragmented up to 20%, and the fragmentation of the type C high = 20% <50%, fragmentation-type embryos = 50% D. Check out also increased sperm volume. To calculate the percentage of embryos in each category, which is derived from fertilized oocyte 2PN. View sperm volume medical scam. Hai also cut normal (type A, the C and B), 2 cell, as 3, were classified depending on the speed of development - from eight-cell stage embryos or cell 4 or 5. To high for you to enter the (fragmentation <50%) has been replaced by the uterine cavity approximately 48 ICSI hours after fertilization or a C. (Only for type B or type) were frozen on day 3 days or 2 high excess of good quality.
Embryo transfer policy has been following. Embryos of the highest quality, (which replaces the three depending on the two), or embryo transfer elective (quality morphological them always from. Staessen, one thousand nine hundred and ninety-three ), preferably in vitro fertilization of conventional (processing procedure of the same derived from or ICSI). The purpose of the case failed pregnancy occurs, as was to continue treatment (IVF), a choice between embryos and IVF and ICSI quality similar embryonic procedure the most expensive complex and at least if it is allowed, the priority was given to the embryo IVF. Rise of serum HCG, since 11 years after embryo transfer showed a two times in a row from the date of pregnancy. Clinical pregnancy is defined by the presence of a gestational sac by ultrasonography after approximately seven weeks of pregnancy. View less sperm volume. Has been defined as the sum of the fetal heart rate of the total number of embryos per implantation rate has been replaced.Parameters for the evaluation
The following parameters were used to compare the efficiency of conventional IVF and ICSI. Fertilization 2PN rate, the insemination after 44 hours from, 42 1 PN = 3 PN rate, after the injection, in a few second day quality and speed of morphological development of the embryo, and (3 or day 2) the number of frozen embryos of embryo transfer to the total number of frozen embryo transfer or. Fertilization rate was calculated as the number of 2PN oocytes retrieved compared to the number of cumulus, always oocyte complexes. There is a possibility as the origin of the embryos for transfer was not a randomized, whatever conclusion is not drawn about the pregnancy.Statistical analysis
Wilcoxon signed rank test for paired data was used to compare the above parameters in sibling oocytes. The Mann-Whitney U test has been used to compare the results among a group of patients with fertilization after the group of patients with in vitro fertilization and traditional. View reduced sperm volume, without fertilization after in vitro fertilization traditional. All tests were carried out at 5% significance level.
All patients before providing a sample of their semen, abstinence was observed 3 to 5 days. See increase volume of sperm. In terms of macroscopic aspects, all semen samples, showed normal liquefaction, five were found slightly to moderately abnormal viscosity of 20. (Oocyte retrieval, retrieve eggs) and fresh semen samples of treatment (from outpatient clinics) is the concentration of sperm are shown in Table I, both the results of basic sperm parameters of fresh semen sample diagnostic revealed a wide range of opportunities. Check out also ways to increase sperm volume. After taking the amount of semen to consider the same observation was made for total sperm count. Average exercise was higher in semen samples of six patients for preliminary assigned to the study to the date of acquisition was only as eggs (range from 8 to 38) type of movement of> 5% of their spare semen percentage of sample types. In search of the oocyte, however, all patients showed a type of exercise maximum of 5%. Was similar in both situations (type A + B) a percentage of the total number of cell motility gradually. <In the case of 10 and 8 respectively show the 5% normal morphology, meaning the percentage of normal morphology at the time of oocyte retrieval and outpatient care were similar. Check out also how to increase sperm volume naturally.
Characteristics (T, egg retrieval) of fresh semen samples of diagnostic treatment (D, from outpatient clinics) and from 20 patients
IgG and IgA tests of anti-sperm antibodies, were performed on semen samples for diagnosis. 2, while it has 100% of their sperm were bound to the antibody IgG, IgG antibody, in patients 18/20 was not observed. One patient was only IgA antibodies (96%). Two patients had a (white blood cells) peroxidase positive round cells of 1.5 10 6 / ml from 1.Fertilization and embryo quality
The average number of oocytes complex 14. Check out also increase sex drive and sperm volume.7 was the average value of 7.4 oocyte that is processed by 7.3 oocytes are treated with IVF and ICSI - cumulus for each patient. Despite the infusion mature only, oocytes of metaphase II, the percentage of = 3PN per cycle and 2PN, 1PN is cumulus always get - has been calculated as a percentage of the number of (II table) oocyte complex. You maybe interested in foods to increase sperm volume. This is not an egg ICSI, will be checked for egg IVF only as the maturity of the oocyte are considered to be the only way to make valid comparisons, on the day of oocyte retrieval . Fertilization rate after in vitro fertilization ICSI (average of 22.9% per cycle) (63.4%, P <0.001) was much lower than the fertilization rate after. 2PN fertilization is most pronounced between always followed the ICSI, 10 points (50%) of 20 cycles, in the conventional IVF, fertilization was the observation of a complete failure. View decreased sperm volume. Patients (n = 10) showed a fertilization rate reasonable 45.7% after in vitro fertilization did not differ significantly from the 59.4% obtained after ICSI for patients with the same 10 fertilization after in vitro fertilization . The proportion of 1PN = 3 PN, for both treatment procedures were similar. View goji berry sperm volume.
Fertilization rate after IVF and ICSI (20 cycles)
Comparison of quality of embryos derived from ICSI and IVF as was observed after in vitro fertilization in 10 cases fertilization at all, based on the nine cycle, just another patient after in vitro fertilization did not disconnect, fertilization only had oocytes. In terms of morphological quality embryos were divided into four categories. Percentage of the total cleavage was the number of portable Hai (is to enter a C max) as a percentage of the number of (III table) oocytes were fertilized. Calculated on the basis of the patient, the percentage of embryos of each morphological class, there was no significant difference between conventional IVF and ICSI. Considering the speed of development, the embryo is ICSI insemination after 44 hours from 42 In addition, we have also been developed after injection than the high in vitro fertilization. See sperm volume enhancer. Percentage of 2-cell stage embryos were significantly higher for ICSI in the morning than in vitro fertilization and embryo transfer (P <0.05).
From 42 to 44 hours after conventional IVF and ICSI of the quality of embryos (9 cycles)
Always has been replaced as the embryo of the highest quality, from the treatment of both, derived from in vitro fertilization, to evaluate the relationship between the injection and non-therapeutic procedures, or from ICSI is not possible. All 20 patients were exchanged at least one embryo. (N = 16) is received (2.1 per patient) High ICSI only for two reasons: most patients: Yes ICSI is developing more sophisticated, at the time of embryo transfer often fertilized embryos in vitro none in 10 cycles, and were not available shows the stage. Two patients, two patients in vitro fertilized embryos (2.0 per patient) were given a transfer of (2.5 per patient) High in vitro fertilization and ICSI together. The result is a point (P <0.001) the total number of embryos a significant difference was replaced between IVF and ICSI, and replaced by the total number of embryos, (P = 0.001, IV table) in terms of the frozen continued. 11 cases (55%) showed a positive HCG, one, there was a biochemical pregnancy, another pregnancy has become the initial miscarriage. Of the nine patients, seven have received only ICSI embryos and ongoing clinical pregnancy (45% per embryo transfer). Implantation rate, the rate of continuous infusion was 30% beyond 20 weeks of pregnancy, was 37%.
Embryo transfer, pregnancy rate and implantation rate (20 cycles)
In 10 cycles, fertilization is not followed by in vitro fertilization treatment for traditional egg at all. Characteristics of the cycle and / or the patient (FERT +, n = 10) and without (FERT-, n = 10) after fertilization in vitro fertilization, therefore, were compared (V table). For most parameters, significant differences were observed between patient populations, regardless of the presence or absence of fertilization after conventional in vitro fertilization. Check out also increase sperm fluid volume. The group had a tendency + sperm concentration is higher in the FERT, this was due to include the case of quite a few at a concentration of> 100 / ml 10 6. The difference between the total sperm count when considering the date of acquisition of the oocyte (sperm concentration volume) is (P = 0.05, V table) became more pronounced important. Usually in the form of a percentage of fresh semen, FERT-. Groups and was comparable to FERT +, the percentage of progressive motility (+ B-type or type) The total number of cell motility, however, the group FERT (P = 0.05, V table) was higher in the group compared to FERT + gradually. Showed a (normal form = 5%) in combination with severe teratozoospermia percentage motility of type 3 out of 10 in the 5 + and FERT-group of 10 patients who have low FERT.
Regardless of the presence or absence of (FERT +) after conventional in vitro fertilization. Check out also increase sperm fluid volume, comparison of patient and cycle characteristics fertilization (FERT-)
The quality of the fractionation sperm last, after prepared by density gradient centrifugation, may be determined by the ratio of sperm (purity of motility) occasionally in the volume fraction to be used for insemination exercise gradually. In our system of droplets, the maximum volume fraction of 5l sperm, in order to avoid further dilution in the droplet, was added to the (25L) each droplet. View healthy sperm volume pregnancy. In this way, the concentration of sperm of the last fraction has been adapted to get the sperm in a volume of up to 5000 from one of 5L motility gradually. If it is required by the fraction of the last with greatest concentration in order to obtain sperm sufficient 5l of the above, before the volume of semen appropriate to be added, first, the volume excess oocytes has been removed from the droplets of medium containing the cells. This is not generally, but does not occur rarely outside the average (eg 10, in this study,) FERT meaning of 6.3l artificial insemination for patient 10 occurred in 5 out of 10 patients (FERT group -group for artificial insemination only 3.5l) + group 10 cases into two groups and from FERT FERT + from. See increase sperm volume naturally. Population FERT (P <0.01, V table) In addition, population groups and FERT + indicates a slow motility of type B mainly in the fraction of the last 5 + cases in the FERT, progress in the last fraction of sperm even more as against 8 out of 10 cases showed a significantly higher proportion of physical exercise, in the FERT group. View vitamins that increase sperm volume.
Between the two groups was not observed in the 2PN rate difference is obtained after ICSI. Pregnancy rate if you are allowed to transfer a large selection of embryos for higher number of embryos available is high, was higher in the group FERT +.
Selection is based on the quality of the semen of male partner often support playback treatment (ICSI IUI, or IVF,) most appropriate for a couple of the individual. ICSI is, otherwise, in the case of serious male infertility would be impossible and treatment of their own gametes is very efficient. The success of ICSI, however, have led to the abuse of this more invasive procedure without a doubt. Obvious reason for this is certainly completely avoid fertilization failure. You maybe interested in decrease in sperm volume. risk of failure of fertilization after conventional in vitro fertilization. Check out also increase sperm fluid volume normozoospermic semen since the introduction of in vitro fertilization, was certainly frustrating element. Unexplained lack of fertilization after in vitro fertilization is observed in approximately 8% of the cycle. According to several studies, this feature shows a low recurrence rate ( from Molley, 1,991 ; from Ben-Shlomo, 1992. ; . Lipitz et al, 1 993 , one thousand nine hundred and ninety-four ). According to another report, a certain phenomenon repetitive show (not just the failure of total fertilization after in vitro fertilization, the underlying rate of fertilization is very low possible, the pathology of the oocyte and sperm undiagnosed . Calderon et al, 1995 ; . Roest et al, 1 998 ). After ICSI, fertilization failure (2.8%) did not occur in rare cases, cases are repeated in only 15% ( Liu et al, 1 995 ; from Vandervorst, 1,997 ; . Flaherty et al, 1998 ). Treatment failed because of a combination of IVF and ICSI in sibling oocytes, in order to avoid or reduce the percentage of fertilization cycle, has been advised in the literature. From Yang: Several studies have compared the conventional IVF and ICSI for tubal infertility and unexplained or conflicting results. While finding the fertilization rate similar others n = 22 (1996), but reported a fertilization rate similar without failure in the (n = 13) series with small cycle, series big cycle of each (22% fertilization failure after in vitro fertilization, of 11% from Aboulghar, 1996 is from, n = 70 Lewis et al, 1997 ).
The difference between IVF and ICSI is more pronounced in the case of male subfertility. In patients with the boundary semen, ICSI is a fertilization rate and the absence of fertilization failure (which shows a better performance than the conventional in vitro fertilization. Check out also increase sperm fluid volume in terms of et al. Aboulghar, 1 995 , one thousand nine hundred and ninety-six , Calderon et al, 1995 ). Aboulghar et al is a reasonable quality of semen has been reported (very high regard, reported a 50% failure rate of fertilization after in vitro fertilization Aboulghar et al, 1995 ). Calderon et al, in fact, IVF traditional (as in patients should be considered untreatable by, a small number of sperm with triple defect of single, double or Calderon et al, 1995 ). Each of the categories defined defect of sperm, of his three, one patient does not have the sperm motility is an indication opposition clear for in vitro fertilization traditional, gradually at least not. Check out also improve sperm volume. Argues that for critical analysis of the data that has been reported that such statements, and to emphasize the variation in the interpretation of the quality of semen `boundary between the groups. Teratozoospermia, IVF after led to the extreme difference between the fertilization rate (76% against 15% of ICSI IVF) ICSI, reported a higher risk of fertilization failure (eg a 11/18) with respect to in vitro fertilization and has been ( and his colleagues Payne, 1994 .) this problem is in vitro fertilization partially (presumably be resolved by the procedure of `concentration insemination high" for more information from the Ord, 1993. ; . from Hall, 1995 ) is (in the left () normal morphology <5% of cases of severe teratozoospermia from Fishel et al, 1,995 ).
For asthenozoospermia, comparison of the control of conventional IVF and ICSI has not been reported yet. In this study, asthenozoospermia, it rapid (type A) sperm in the sample gradually to 5% of fresh semen = motility is defined as the percentage is very low. See naturally increase sperm volume. Under various conditions, but may impair the motility of sperm, the pathogenesis of asthenozoospermia is often unknown. The anti-sperm antibodies, infection of the reproductive organs, anejaculation, high semen viscosity, an extended period of ultra-fine defects and metabolic disorders are the most common cause (some from Vandervorst, 1,997 ). In this study, some patients, white blood cell count was slightly elevated and / or antisperm antibodies and / or viscosity of semen and high, (May 20), and (February 20) (February 20) was. Although these parameters may interfere with the results of fertilization after in vitro fertilization or conventional, impair motility, patients showing these defects have been distributed in more than (V table) FERT + group and FERT equally .
The most striking observation is the high failure rate of fertilization and fertilization rate after in vitro fertilization very low in comparison of conventional IVF and ICSI in this study population. Once, the conventional in vitro fertilization. Check out also increase sperm fluid volume and 2PN fertilization has taken place, however, that speed was considerably higher (45.7%), the trend is lower than that (59.4%) 2PN rate after ICSI for patients with the same 10 people showed. See sperm volume enhancer for men. Observed rate of 2PN that was 59.4% and 67.5% for a group of FERT + and FERT, respectively, are impaired in population this study, not an event intrusion of post-rather, oocytes after in vitro fertilization traditional . You maybe interested in foods that increase sperm and volumeafter ICSI through investment into the cell, shows that the penetration rate. (III table), the ICSI technique itself is specific to the difference between the growth rate of embryos in vitro fertilization and ICSI. Nagy et al. ICSI showed that pronuclear formation (1998) occurred after 4 hours earlier than after in vitro fertilization for mechanical bypass of some barriers. This is the reason why in vitro fertilized embryos were still at the stage of 2-cell on the morning of day 2 embryos than ICSI. In this study, population was achieved fertilization after in vitro fertilization is due to cleavage stage after embryonic ICSI at the time of embryo transfer was replaced (36/43) In addition, even at high ICSI more (FERT +), rather than morphological differences in quality. High pregnancy rate was obtained. Pregnancy was obtained in four patient populations without fertilization after in vitro fertilization if ICSI has not been used, did not occur. Was rescued by ICSI and IVF legacy applications for these patients, have been combined cycle at least.
Half of the patients as obtained fertilization only in the other half with both the IVF ICSI and ICSI, two groups have been carefully considered. Semen samples of high viscosity, high anti-sperm antibodies or white blood cells, was distributed evenly in the two populations. After meeting, out of 10 patients with fertilization, fertilization after in vitro fertilization, each of these defects IVF is similar to defects and the original three, one of five out of 10 patients without fertilization after in vitro fertilization, It may interfere with the 5. In terms of basic sperm parameters, significant differences were observed a number of progressive motile sperm and total sperm number of the date of acquisition of oocyte. Counts in the range of 10 6 ~ 12.4 10 6 4.2 at oocyte retrieval for in FERT FERT group exercise gradually, 5.0 10 6 ~ 83.0 10 6 ~ + group. Seems to be the quality of the sperm fraction prepared for the injection and artificial insemination was superior in terms of motility was obtained for patients in fertilization (FERT +) for both therapeutic procedures more relevant.1996VCL
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<5ICSI Of paramount importance seems to be the quality of the inseminating sperm fraction, with a higher rate of immotile spermatozoa and predominantly slow progressive motility in patients without fertilization after IVF. As the differences between the two subgroups were not clear-cut, it still remains to detect the underlying factors which might have a prognostic value for the success of conventional IVF. So far, however, these data argue for the application of a diagnostic combined conventional IVF/ICSI treatment cycle for male subfertility characterized by low or no rapid progressive motility, in order to avoid complete fertilization failure with conventional IVF.
The authors wish to thank the clinical, paramedical and laboratory staff of the Centre for Reproductive Medicine. Furthermore, we are grateful to Mr Frank Winter of the Language Education Centre of our university for correcting the manuscript. This work was supported by grants from the Fund for Scientific Research Flanders.
- European Society of Human Reproduction and Embryology